The Slo1 C-Tail Domain Confers Cholesterol-Sensitivity to Arterial Smooth Muscle BK Channels
نویسندگان
چکیده
منابع مشابه
Gating Mechanism of BK (Slo1) Channels
Large conductance calciumand voltage-activated K channels (Slo1), also referred to as “BK” or “maxi K” channels because of their high single channel conductance (250–300 pS in symmetrical 150 mM KCl), are widely distributed in many different tissues (Kaczorowski et al., 1996). A signature feature of BK channels, in addition to their high K selectivity and conductance, is that they are activated...
متن کاملSingle-channel kinetics of BK (Slo1) channels
Single-channel kinetics has proven a powerful tool to reveal information about the gating mechanisms that control the opening and closing of ion channels. This introductory review focuses on the gating of large conductance Ca(2+)- and voltage-activated K(+) (BK or Slo1) channels at the single-channel level. It starts with single-channel current records and progresses to presentation and analysi...
متن کاملEthanol sensitivity of BK(Ca) channels from arterial smooth muscle does not require the presence of the beta 1-subunit.
Ethanol inhibition of large-conductance, Ca(2+)-activated K(+) (BK(Ca)) channels in aortic myocytes may contribute to the direct contraction of aortic smooth muscle produced by acute alcohol exposure. In this tissue, BK(Ca) channels consist of pore-forming (bslo) and modulatory (beta) subunits. Here, modulation of aortic myocyte BK(Ca) channels by acute alcohol was explored by expressing bslo s...
متن کاملSlo1 Tail Domains, but Not the Ca2+ Bowl, Are Required for the β1 Subunit to Increase the Apparent Ca2+ Sensitivity of BK Channels
Functional large-conductance Ca(2+)- and voltage-activated K(+) (BK) channels can be assembled from four alpha subunits (Slo1) alone, or together with four auxiliary beta1 subunits to greatly increase the apparent Ca(2+) sensitivity of the channel. We examined the structural features involved in this modulation with two types of experiments. In the first, the tail domain of the alpha subunit, w...
متن کاملTransduction of voltage and Ca2+ signals by Slo1 BK channels.
Large-conductance Ca2+ -and voltage-gated K+ channels are activated by an increase in intracellular Ca2+ concentration and/or depolarization. The channel activation mechanism is well described by an allosteric model encompassing the gate, voltage sensors, and Ca2+ sensors, and the model is an excellent framework to understand the influences of auxiliary β and γ subunits and regulatory factors s...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2011
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2010.12.3370